Answer:
In a negative stain, the dye stains the background, forming a silhouette around the unstained organism.
Explanation:
In this kind of staining, heat fixation is not a requirement since there is less distortion or shrinkage of cells.
This staining is used to accentuate the capsule surrounding bacteria and yeasts.
In negative staining, nigrosin and india ink are used in the process.
